human Integrin Alpha 5 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-human Integrin Alpha 5 (fibronectin receptor α, VLA-5α, CD49e)
Catalog Number: 14-0496
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioSAM-1 (SAM-1, SAM1)
Isotype: Mouse IgG1

Product Options

Price

Cat. No.

Size

To view relevant pricing,
please select your country
and resume browsing.

14-0496-80

25 ug

14-0496-82

100 ug

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human Integrin Alpha 5 (eBioSam-1) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0496	Affinity Purified anti-human CD49e (Integrin alpha 5)	N/A	N/A	FC IHC IP
13-0496	Biotin anti-human CD49e (Integrin alpha 5)	N/A	N/A	FC
53-0496	Alexa Fluor® 488 anti-human CD49e (Integrin alpha 5)	488	519	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioSAM-1 monoclonal antibody reacts with human integrin alpha 5, also known as fibronectin receptor alpha chain, very late activation antigen 5 alpha, and CD49e. Integrins are composed of an alpha chain and a beta chain, which non-covalently associate to form the functional integrin. Integrin heterodimers participate in cell surface-mediated signaling and adhesion functions. Integrin alpha 5 undergoes post-translational cleavage in its extracellular domain to yield disulfide linked light and heavy chains that join with Integrin beta 1 (CD29) to form the fibronectin receptor, also known as the very late activation antigen-5 (VLA-5) complex. Integrin alpha 5 is expressed on thymocytes, T cells, monocytes, platelets, early B cells, and activated B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioSAM-1 (SAM-1, SAM1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.

Applications Tested

This eBioSAM-1 (SAM-1, SAM1) antibody has been tested by flow cytometric analysis of human PBMCs. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Xiao J, Messinger Y, Jin J, Myers DE, Bolen JB, Uckun FM. Signal transduction through the beta1 integrin family surface adhesion molecules VLA-4 and VLA-5 of human B-cell precursors activates CD19 receptor-associated protein-tyrosine kinases. J Biol Chem. 1996 Mar 29;271(13):7659-64. (SAM-1, FC, PubMed)

Grassi F, Dezutter-Dambuyant C, McIlroy D, Jacquet C, Yoneda K, Imamura S, Boumsell L, Schmitt D, Autran B, Debre P, Hosmalin A. Monocyte-derived dendritic cells have a phenotype comparable to that of dermal dendritic cells and display ultrastructural granules distinct from Birbeck granules. J Leukoc Biol. 1998 Oct;64(4):484-93. (SAM-1, FC, IHC, PubMed)

Santoni G, Birarelli P, Hong LJ, Gamero A, Djeu JY, Piccoli M. An alpha 5 beta 1-like integrin receptor mediates the binding of less pathogenic Candida species to fibronectin. J Med Microbiol. 1995 Nov;43(5):360-7. (SAM-1, FC, PubMed)

Theodore PR, Simon AR, Warrens AN, Sackstein R, Sykes M. Porcine mononuclear cells adhere to human fibronectin independently of very late antigen-5: implications for donor-specific tolerance induction in xenotransplantation. Xenotransplantation. 2002 Jul;9(4):277-89. (SAM-1, FA, PubMed)

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control