human/mouse CD49f antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-human/mouse CD49f (Integrin alpha 6)
Catalog Number: 14-0495
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioGoH3 (GoH3)
Isotype: Rat IgG2a, κ

Product Options		Price
Cat. No.	Size	To view relevant pricing, please select your country and resume browsing.
14-0495-81	50 ug
14-0495-82	100 ug
14-0495-85	500 ug

Staining of normal human peripheral blood cells with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified anti-hu/mo CD49f (eBioGoH3) (colored histogram) followed by PE anti-rat IgG (cat. 12-4822). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0495	Affinity Purified anti-human/mouse CD49f (Integrin alpha 6)	N/A	N/A	FC IH/F IP
13-0495	Biotin anti-human/mouse CD49f (Integrin alpha 6)	N/A	N/A	FC IH/F
12-0495	Phycoerythrin (PE) anti-human/mouse CD49f (Integrin alpha 6)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex; CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The eBioGoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioGoH3 (GoH3) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.

Applications Tested

This eBioGoH3 (GoH3) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sonnenberg A, Daams H, Van der Valk MA, Hilkens J, Hilgers J. Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin. J Histochem Cytochem. 1986 Aug;34(8):1037-46. (GoH3, FC, IH/F PubMed)

Aumailley M, Timpl R, Sonnenberg A. Antibody to integrin alpha 6 subunit specifically inhibits cell-binding to laminin fragment 8. Exp Cell Res. 1990 May;188(1):55-60. (GoH3, FA, PubMed)

Ambrose HE, Wagner SD. Alpha6-integrin is expressed on germinal centre B cells and modifies growth of a B-cell line. Immunology. 2004 Apr;111(4):400-6. (GoH3, FC, PubMed)

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