Product Information

Contents: Affinity Purified anti-mouse CD49d (Integrin α₄)
Catalog Number: 14-0492
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: R1-2
Isotype: Rat IgG2b, κ

Surface staining of mouse splenocytes with anti-mouse CD49d (R1-2) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0492	Affinity Purified anti-mouse CD49d (Integrin alpha 4)	N/A	N/A	FA FC IHC IP
16-0492	Functional Grade* Purified anti-mouse CD49d (Integrin alpha 4)	N/A	N/A	FC
13-0492	Biotin anti-mouse CD49d (Integrin alpha 4)	N/A	N/A	FC
11-0492	FITC anti-mouse CD49d (Integrin alpha 4)	488	518	FC
12-0492	PE anti-mouse CD49d (Integrin alpha 4)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin α₄ subunit. The complex of CD49d non-covalently associated with integrin β₁ (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular ADdressin (MAdCAM-1).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The R1-2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. R1-2 has also been reported in in vitro functional assays. (Please use Functional Grade purified R1-2, cat. 16-0492, in functional assays.)

Applications Tested

The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Holzmann, B., B. W. McIntyre, et al. (1989). "Identification of a murine Peyer's patch--specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4 alpha." Cell 56(1): 37-46.
Neuhaus, H., M. C. Hu, et al. (1991). "Cloning and expression of cDNAs for the alpha subunit of the murine lymphocyte-Peyer's patch adhesion molecule." J Cell Biol 115(4): 1149-58.

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