Product Information

Contents: Affinity Purified anti-mouse CD49b (Integrin α₂)
Catalog Number: 14-0491
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: HMa2
Isotype: Armenian Hamster IgG

Staining of BALB/c splenocytes with 0.25 μg of Purified Armenian Hamster IgG Iso Cntrl (cat. 14-4888) (open histogram) or 0.25 μg of Purified anti-mouse CD49b (HMa2) (colored histogram) followed by FITC Anti-Armenian Hamster IgG (cat. 11-4111). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0491	Affinity Purified anti-mouse CD49b (Integrin alpha 2)	N/A	N/A	FA FC IHC IP
16-0491	Functional Grade* Purified anti-mouse CD49b (Integrin alpha 2)	N/A	N/A	FA FC
13-0491	Biotin anti-mouse CD49b (Integrin alpha 2)	N/A	N/A	FC
11-0491	FITC anti-mouse CD49b (Integrin alpha 2)	488	518	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HMα2 monoclonal antibody reacts with mouse CD49b, the 150 kDa integrin α₂ subunit. The complex of CD49b non-covalently associated with integrin β₁ (CD29), also known as VLA-2, is a receptor for collagen and laminin. This complex is expressed by some CD4⁺ T cells, IEL, NK cells, platelets and epithelial cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HMα2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. It has also been reported for use in in vitro functional assays (Please use Functional Grade purified HMa2, cat. 16-0491, in functional assays.)

Applications Tested

The HMα2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Miyake, S., T. Sakurai, et al. (1994). "Identification of collagen and laminin receptor integrins on murine T lymphocytes." Eur J Immunol 24(9): 2000-5.
Noto, K., K. Kato, et al. (1995). "Identification and functional characterization of mouse CD29 with a mAb." Int Immunol 7(5): 835-42.

Related Products

Cat. 11-4111  FITC Anti-Armenian Hamster IgG
Cat. 13-4113  Biotin Anti-Armenian Hamster IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 16-4444  Functional Grade Purified Armenian Hamster IgG Isotype Control (clone n/a)
Cat. 14-4888  Affinity Purified Armenian Hamster IgG Isotype Control (clone eBio299Arm)