Product Information

Contents: Affinity Purified anti-human CD46
Catalog Number: 14-0469
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 8E2
Isotype: Mouse IgG1, κ

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human CD46 (8E2) (colored histogram) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (cat. 12-4012). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0469	Affinity Purified anti-human CD46	N/A	N/A	FC IHC(Paraffin) IP WB
12-0469	Phycoerythrin (PE) anti-human CD46	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 8E2 monoclonal antibody reacts with human CD46 (membrane cofactor protein). CD46 is a transmembrane glycoprotein consisting of four regularly expressed isoforms, giving an electrophoretic profile of two heterogeneous protein species of 51-58 kDa ("lower band") and 59-68 kDa ("upper band"). CD46 is expressed on the majority of nucleated cells, and functions as a regulator of complement function by binding complement components C3b and C4b, which serves to protect the host from autologous complement attack. CD46 has been called a "pathogen magnet" as it is known to play a role in the entry of several pathogens including strains of measles virus, human herpes virus type 6, group A streptococci and Neisseria.

Cross-linking TCR on naïve human peripheral blood CD4+ T cells together with CD46, rather than CD28, induces more robust proliferation than achieved with stimulation by the costimulatory molecule CD28, as well as other qualitative differences. There is evidence to suggest that stimulation of TCR and CD46 activates natural CD4+CD25+ T regs, which are non-proliferating, but produce high amounts of granzyme A, and kill activated CD4+, CD8+, CD14+ monocytes, immature dendritic cells and mature dendritic cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 8E2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) (non-reducing), and immunohistology staining of paraffin embedded tissue sections.

Applications Tested

This 8E2 antibody has been tested by flow cytometric analysis of nomal human peripheral blood. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Adams EM, Brown MC, Nunge M, Krych M, Atkinson JP. Contribution of the repeating domains of membrane cofactor protein (CD46) of the complement system to ligand binding and cofactor activity. J Immunol. 1991 Nov 1;147(9):3005-11.

Astier A, Trescol-Biemont MC, Azocar O, Lamouille B, Rabourdin-Combe C. Cutting edge: CD46, a new costimulatory molecule for T cells, that induces p120CBL and LAT phosphorylation. J Immunol. 2000 Jun 15;164(12):6091-5.

Grossman WJ, Verbsky JW, Barchet W, Colonna M, Atkinson JP, Ley TJ. Human T regulatory cells can use the perforin pathway to cause autologous target cell death. Immunity. 2004 Oct;21(4):589-601.

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