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Product Information

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Contents: Affinity Purified anti-human CD45RA Catalog Number: 14-0458 Sizes: 25 ug, 100 ug Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: HI100 Isotype: Mouse IgG2b, κ HLDA No.: IV N906

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (open histogram) or 0.25 μg of Purified anti-human CD45RA (HI100) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0458	Affinity Purified anti-human CD45RA	N/A	N/A	FC  IHC
13-0458	Biotin anti-human CD45RA	N/A	N/A	FC
11-0458	FITC anti-human CD45RA	488	518	FC
12-0458	PE anti-human CD45RA	488	575	FC
15-0458	PE-Cy5 anti-human CD45RA	488	670	FC
17-0458	Allophycocyanin (APC) anti-human CD45RA	633	660	FC
25-0458	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45RA	488	760	FC
45-0458	PerCP-Cy5.5 anti-human CD45RA (PE-Cy5.5 replacement)	488	690	FC
93-0458	eFluorTM 605NC anti-human CD45RA	350-500	605	FC
48-0458	eFluorTM 450 anti-human CD45RA (Pacific Blue® replacement)	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4⁺ peripheral T lymphocytes, CD8⁺ peripheral T lymphocytes, and B cells. The CD45RA⁺ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The HI100 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

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Applications Tested

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The HI100 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

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Related Products

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Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4732  Affinity Purified Mouse IgG2b Isotype Control
Cat. 11-9979  FITC anti-human CD45RA (clone JS-83)
Cat. 12-9979  PE anti-human CD45RA (clone JS-83)
Cat. 13-9979  Biotin anti-human CD45RA (clone JS-83)
Cat. 14-9979  Affinity Purified anti-human CD45RA (clone JS-83)
Cat. 15-9979  PE-Cy5 anti-human CD45RA (clone JS-83)