Product Information

Contents: Affinity Purified anti-human CD45RA
Catalog Number: 14-0458
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: HI100
Isotype: Mouse IgG2b, κ
HLDA No.: IV N906

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (open histogram) or 0.25 μg of Purified anti-human CD45RA (HI100) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0458	FITC anti-human CD45RA	488	518	FC
12-0458	PE anti-human CD45RA	488	575	FC
15-0458	PE-Cy5 anti-human CD45RA	488	670	FC
17-0458	Allophycocyanin (APC) anti-human CD45RA	633	660	FC
25-0458	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45RA	488	760	FC
57-0458	Pacific Blue® anti-human CD45RA	402	421	FC
45-0458	PerCP-Cy5.5 anti-human CD45RA	488	690	FC
93-0458	eFluor^TM 605^NC anti-human CD45RA	350-500	605	FC
14-0458	Affinity Purified anti-human CD45RA	N/A	N/A	FC IHC
13-0458	Biotin anti-human CD45RA	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4⁺ peripheral T lymphocytes, CD8⁺ peripheral T lymphocytes, and B cells. The CD45RA⁺ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HI100 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested

The HI100 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4732    Affinity Purified Mouse IgG2b Isotype Control
Cat. 11-9979    FITC anti-human CD45RA (clone JS-83)
Cat. 12-9979    PE anti-human CD45RA (clone JS-83)
Cat. 13-9979    Biotin anti-human CD45RA (clone JS-83)
Cat. 14-9979    Affinity Purified anti-human CD45RA (clone JS-83)
Cat. 15-9979    PE-Cy5 anti-human CD45RA (clone JS-83)