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Product Information


Contents: Affinity Purified anti-human CD45RA
Catalog Number: 14-0458
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: HI100
Isotype: Mouse IgG2b, κ
HLDA No.: IV N906
 
 
data image 1
Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (open histogram) or 0.25 μg of Purified anti-human CD45RA (HI100) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis. 

Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
14-0458 Affinity Purified anti-human CD45RA N/A N/A FC  IHC 
13-0458 Biotin anti-human CD45RA N/A N/A FC 
11-0458 FITC anti-human CD45RA 488 518 FC 
12-0458 PE anti-human CD45RA 488 575 FC 
15-0458 PE-Cy5 anti-human CD45RA 488 670 FC 
17-0458 Allophycocyanin (APC) anti-human CD45RA 633 660 FC 
25-0458 Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45RA 488 760 FC 
45-0458 PerCP-Cy5.5 anti-human CD45RA (PE-Cy5.5 replacement) 488 690 FC 
93-0458 eFluorTM 605NC anti-human CD45RA 350-500 605 FC 
48-0458 eFluorTM 450 anti-human CD45RA (Pacific Blue® replacement) 405 450 FC 
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description


The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.


Applications Reported


For research use only, not for diagnostic or therapeutic use. The HI100 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.


Applications Tested


The HI100 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.


References



Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.


Related Products


Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4732  Affinity Purified Mouse IgG2b Isotype Control
Cat. 11-9979  FITC anti-human CD45RA (clone JS-83)
Cat. 12-9979  PE anti-human CD45RA (clone JS-83)
Cat. 13-9979  Biotin anti-human CD45RA (clone JS-83)
Cat. 14-9979  Affinity Purified anti-human CD45RA (clone JS-83)
Cat. 15-9979  PE-Cy5 anti-human CD45RA (clone JS-83)



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