Product Information

Contents: Affinity Purified anti-human CD45RO
Catalog Number: 14-0457
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: UCHL1
Isotype: Mouse IgG2a, κ
HLDA No.: IV N31

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG2a, K Iso Cntrl (cat. 14-4724) (open histogram) or 0.25 μg of Purified anti-human CD45RO (UCHL1) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0457	Affinity Purified anti-human CD45RO	N/A	N/A	FC IHC
11-0457	FITC anti-human CD45RO	488	518	FC
12-0457	PE anti-human CD45RO	488	575	FC
15-0457	PE-Cy5 anti-human CD45RO	488	670	FC
17-0457	Allophycocyanin (APC) anti-human CD45RO	633	660	FC
95-0457	eFluor^TM 650^NC anti-human CD45RO	350-500	650	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The UCHL1 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested

The UCHL1 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4724  Affinity Purified Mouse IgG2a, K Isotype Control