Product Information

Contents: Affinity Purified anti-mouse CD45RC
Catalog Number: 14-0456
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: GL24
Isotype: Rat IgM, κ

Staining of BALB/c splenocytes with 0.25 μg of Purified Rat IgM Iso Cntrl (cat. 14-4341) (open histogram) or 0.25 μg of Purified anti-mouse CD45RC (GL24) (colored histogram) followed by PE anti-rat IgM (HIS40) (cat. 12-0990). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0456	Affinity Purified anti-mouse CD45RC	N/A	N/A	FC IP
13-0456	Biotin anti-mouse CD45RC	N/A	N/A	FC
11-0456	FITC anti-mouse CD45RC	488	518	FC
12-0456	PE anti-mouse CD45RC	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The GL24 monoclonal antibody reacts with the exon C-dependent isoform of mouse CD45. This CD45 isoform is expressed by peripheral B cells and a population of CD4⁺ T cells at high levels, on a majority of CD8⁺ T cells at intermediate levels and by a subpopulation of mouse thymocytes. CD45 is a protein tyrosine phosphatase and serves an important role in leukocyte signal transduction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The GL24 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested

The GL24 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hathcock, K. S., G. Laszlo, et al. (1992).Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations. J Immunol 148(1): 19-28.

Related Products

Cat. 11-0990  FITC anti-rat IgM (clone HIS40)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4341  Affinity Purified Rat IgM Isotype Control