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Mouse Cellular Antigens Chart

Contents: Affinity Purified anti-mouse CD45RC Catalog Number: 14-0456 Formulation: Phosphate buffer pH 7.2, 500 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: GL24 Isotype: Rat IgM, κ Prices for This Product* Cat. No. Size Price Qty Action 14-0456-81 50 ug $60 14-0456-82 100 ug $85 14-0456-85 500 ug $265 *International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes with 0.25 μg of Purified Rat IgM Iso Cntrl (cat. 14-4341) (open histogram) or 0.25 μg of Purified anti-mouse CD45RC (GL24) (colored histogram) followed by PE anti-rat IgM (HIS40) (cat. 12-0990). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0456	FITC anti-mouse CD45RC	488	518	FC
12-0456	PE anti-mouse CD45RC	488	575	FC
13-0456	Biotin anti-mouse CD45RC	N/A	N/A	FC
14-0456	Affinity Purified anti-mouse CD45RC	N/A	N/A	FC  IP

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The GL24 monoclonal antibody reacts with the exon C-dependent isoform of mouse CD45. This CD45 isoform is expressed by peripheral B cells and a population of CD4⁺ T cells at high levels, on a majority of CD8⁺ T cells at intermediate levels and by a subpopulation of mouse thymocytes. CD45 is a protein tyrosine phosphatase and serves an important role in leukocyte signal transduction.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The GL24 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

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Applications Tested

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The GL24 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Hathcock, K. S., G. Laszlo, et al. (1992).Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations. J Immunol 148(1): 19-28.

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Related Products

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Cat. 11-0990    FITC anti-rat IgM (clone HIS40)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4341    Affinity Purified Rat IgM Isotype Control