Product Information
Contents: Affinity Purified anti-mouse CD45.2
Catalog Number: 14-0454
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles.
Clone: 104
Isotype: Mouse IgG2a, κ
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Staining of C57Bl/6 splenocytes with 0.25 µg of Purified mouse IgG2a isotype control (cat.14-4724) (open histogram), or 0.25 µg Purified anti-mouse CD45.2 (104) followed by FITC anti-mouse IgG (cat. 11-4011) (closed histogram). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite (nm) |
Emit (nm) |
Reported Applications |
| 14-0454 |
Affinity Purified anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
N/A |
N/A |
FC
IH/F
IP
|
| 13-0454 |
Biotin anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
N/A |
N/A |
FC
IH/F
|
| 11-0454 |
FITC anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
488 |
518 |
FC
|
| 12-0454 |
PE anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
488 |
575 |
FC
|
| 17-0454 |
Allophycocyanin (APC) anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
633 |
660 |
FC
|
| 35-0454 |
PE-Cy5.5 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) |
488 |
690 |
FC
|
| 56-0454 |
Alexa Fluor® 700 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 45-0454 |
PerCP-Cy5.5 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) (PE-Cy5.5 replacement) |
488 |
690 |
FC
|
| 47-0454 |
Allophycocyanin-eFluorTM 780 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) (APC-Alexa Fluor® 750 replacement) |
633 |
780 |
FC
|
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 104 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. (Biotinylated 104 is recommended for IHC cat.
13-0454).
Applications Tested
The 104 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 0.5 µg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Shen, F-W. (1981). "Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. In Monoclonal antibodies and T cell Hybridomas; Perspective and technical advances, eds. Hammerling, G.J., U. Hammerling, and J.F. Kearney": 25-31.
Related Products
Cat. 11-4011 FITC Anti-Mouse IgGCat. 13-4013 Biotin Anti-Mouse IgG (clone Polyclonal)Cat. 11-4317 Streptavidin-FITC (Fluorescein isothiocyanate)Cat. 12-4317 Streptavidin-PE (Phycoerythrin)Cat. 17-4317 Streptavidin Allophycocyanin (SA-APC)Cat. 14-4724 Affinity Purified Mouse IgG2a, K Isotype Control