mouse/human CD45R antibody: purified antibody

Contents: Affinity Purified anti-mouse/human CD45R (B220)
Catalog Number: 14-0452
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: RA3-6B2
Isotype: Rat IgG2a, κ
HLDA No.: N/A

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-0452-81	50 ug	$50
14-0452-82	100 ug	$70
14-0452-85	500 ug	$195
14-0452-86	1 mg	$350

*International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 splenocytes with 0.5 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.5 μg of Purified anti-mo/hu B220 (RA3-6B2) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0452	FITC anti-mouse/human CD45R (B220)	488	518	FC
12-0452	PE anti-mouse/human CD45R (B220)	488	575	FC
15-0452	PE-Cy5 anti-mouse/human CD45R (B220)	488	670	FC
17-0452	APC anti-mouse/human CD45R (B220)	633	660	FC
19-0452	Cy5 anti-mouse/human CD45R (B220)	633	670	FC
25-0452	PE-Cy7 anti-mouse/human CD45R (B220)	488	760	FC
30-0452	May be discontinued; see superior product Alexa Fluor® 700 Allophycocyanin-Cy5.5 (APC-Cy5.5) anti-mouse/human CD45R (B220) (Alexa Fluor® 700 Equivalent)	633	690	FC
35-0452	PE-Cy5.5 anti-mouse/human CD45R (B220)	488	690	FC
36-0452	Functional Grade* Biotin anti-mouse/human CD45R (B220)	N/A	N/A	FC
51-0452	Alexa Fluor® 647 anti-mouse/human CD45R (B220)	633	668	FC
53-0452	Alexa Fluor® 488 anti-mouse/human CD45R (B220)	488	519	FC
56-0452	Alexa Fluor® 700 anti-mouse/human CD45R (B220) (APC-Cy5.5 Equivalent)	633	723	FC
57-0452	Pacific Blue® anti-mouse/human CD45R (B220)	402	421	FC
27-0452	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse/human CD45R (B220) (APC-Cy7 Equivalent)	633	779	FC
45-0452	PerCP-Cy5.5 anti-mouse/human CD45R (B220)	488	690	FC
93-0452	eFluor^TM 605^NC anti-mouse/human CD45R ( B220)	350-500	605	FC
14-0452	Affinity Purified anti-mouse/human CD45R (B220)	N/A	N/A	FC IH/F IHC(Paraffin) IP
16-0452	Functional Grade* Purified anti-mouse/human CD45R (B220)	N/A	N/A	FA FC
13-0452	Biotin anti-mouse/human CD45R (B220)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RA3-6B2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. RA3-6B2 has also been reported in functional studies of B cells. (Please use Functional Grade purified RA3-6B2, cat. 16-0452, in functional assays.)

Applications Tested

The RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Morse, H. C. d., W. F. Davidson, et al. 1982. Abnormalities induced by the mutant gene Ipr: expansion of a unique lymphocyte subset. J Immunol. 129(6): 2612-5.

Coffman, R. L. 1982. Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev. 69: 5-23.

Schuhmann B, Dietrich A, et al. 2005. A role for brain-derived neurotrophic factor in B cell development. J Neuroimmunol. 163(1-2):15-23. (IHC frozen, PubMed)

Monteith CE, Chelack BJ, et al. 1996. Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues. Can J Vet Res. 60(3):193-8. (IHC frozen and paraffin, PubMed)

Related Products

Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4321    Affinity Purified Rat IgG2a Isotype Control
Cat. 11-4811    FITC Anti-Rat IgG
Cat. 13-4813    Biotin Anti-Rat IgG (clone Polyclonal)