Also known as: Leukocyte Common Antigen, LCA, Ly-5
RUO: For Research Use Only
Staining of C57Bl/6 splenocytes with 0.25 µg of Rat IgG2b Isotype Control Purified (cat. 14-4031) (open histogram) or 0.25 µg of Anti-Mouse CD45 Purified (filled histogram) followed by Anti-Rat IgG FITC (cat. 11-4811). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Mouse CD45 Purified Catalog Number: 14-0451 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Clone: 30-F11 Host/Isotype: Rat IgG2b, κ
Product Options
Cat. No.
Size
Price
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14-0451-81
50 ug
14-0451-82
100 ug
14-0451-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 30-F11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 30-F11 has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified 30-F11 in functional assays.)
Applications Tested
The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.
