rat CD45.2 antibody: purified antibody

Contents: Affinity Purified anti-rat CD45.2
Catalog Number: 14-0450
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: HIS41
Isotype: Mouse IgG1

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-0450-82	100 ug	$95

*International customers: Please contact your distributor for region specific pricing.

Staining of rat splenocytes with 0.06 µg Purified mouse IgG1 isotype control (cat.14-4714) (open histogram) or 0.06 µg Purified anti-rat CD45.2 (HIS41) (colored histogram) followed by FITC anti-mouse IgG (cat.11-4011). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0450	Affinity Purified anti-rat CD45.2	N/A	N/A	FC IH/F
11-0450	FITC anti-rat CD45.2	488	518	FC
12-0450	PE anti-rat CD45.2	488	575	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HIS41 monoclonal antibody reacts with all isoforms of the rat CD45 molecule, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in signaling and activation of lymphocytes. HIS41 reacts with strains expressing the RT7.2 alloantigen (BUF, LOU, and WF), but not strains expressing the RT7.1 alloantigen (ACI, AO, BN, DA, DZB, LEW, MAXX, PVG, SHR, and WAG).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIS41 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The HIS41 antibody has been tested by flow cytometric analysis of LOU rat splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kampinga, J., F.G.M. Kroese, G.H. Pol, D. Opstelten, H.G. Seijen, J.H.A. Boot, B. Roser, P. Nieuwenhuis, and R. Aspinall. 1990. RT7-defined alloantigens in rats are part of the leukocyte common antigen family. Scand. J. Immunol. 31:699-710.
Giezeman-Smits, K. M., A. Gorter, R. L. P. van Vlierberghe, J. D. H. v. Eendenburg, A. M. M. Eggermont, G. J. Fleuren, P. J. K. Kuppen. 1999. The Regulatory Role of CD45 on Rat NK Cells in Target Cell Lysis. J. Immunol. 163:71-76.
Butcher, G.W. 1987. A list of monoclonal antibodies specific for alloantigens of the rat. J. Immunogenet. 14:163-176.

Related Products

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control