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Mouse Cellular Antigens Chart  Human Cellular Antigens Chart

Contents: Affinity Purified anti-mouse/human CD44 Catalog Number: 14-0441 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: IM7 Isotype: Rat IgG2b, κ Prices for This Product* Cat. No. Size Price Qty Action 14-0441-81 50 ug $60 14-0441-82 100 ug $100 14-0441-85 500 ug $220 14-0441-86 1 mg $350 *International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 splenocytes with 0.06 μg Purified Rat IgG2b Iso Cntrl (cat. 14-4331) (open histogram) or 0.06 μg Purified anti-mouse/human CD44 (IM7) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0441	Affinity Purified anti-mouse/human CD44 (Pgp-1, Ly-24)	N/A	N/A	FA  FC  IHC  IP  WB
16-0441	Functional Grade* Purified anti-mouse/human CD44 (Pgp-1, Ly-24)	N/A	N/A	FA  FC
13-0441	Biotin anti-mouse/human CD44 (Pgp-1, Ly-24)	N/A	N/A	FC
11-0441	FITC anti-mouse/human CD44 (Pgp-1, Ly-24)	488	518	FC
12-0441	PE anti-mouse/human CD44 (Pgp-1, Ly-24)	488	575	FC
15-0441	PE-Cy5 anti-mouse/human CD44 (Pgp-1, Ly-24)	488	670	FC
17-0441	APC anti-mouse/human CD44 (Pgp-1, Ly-24)	633	660	FC
25-0441	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse/human CD44 (Pgp-1, Ly-24)	488	760	FC
35-0441	PE-Cy5.5 anti-mouse/human CD44 (Pgp-1, Ly-24)	488	690	FC
56-0441	Alexa Fluor® 700 anti-mouse/human CD44 (Pgp-1, Ly-24) (APC-Cy5.5 Equivalent)	633	723	FC
57-0441	Pacific Blue® anti-mouse/human CD44 (Pgp-1, Ly-24)	402	421	FC
27-0441	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse/human CD44 (Pgp-1, Ly-24) (APC-Cy7 Equivalent)	633	779	FC
45-0441	PerCP-Cy5.5 anti-mouse/human CD44 (Pgp-1, Ly-24)	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.   Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The IM7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistochemical staining and immunoblotting (non-reducing conditions). It has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified IM7, cat. 16-0441, in functional assays.)

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Applications Tested

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The IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Trowbridge, I. S., J. Lesley, et al. 1982.Biochemical characterization and cellular distribution of a polymorphic, murine cell-surface glycoprotein expressed on lymphoid tissues. Immunogenetics 15(3): 299-312.
Lesley, J. and I. S. Trowbridge 1982. Genetic characterization of a polymorphic murine cell-surface glycoprotein. Immunogenetics 15(3): 313-20.
Maiti A, Maki G, Johnson P. TNF-alpha induction of CD44-mediated leukocyte adhesion by sulfation. Science. 1998. Oct 30;282(5390):941-3.

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Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 11-4811    FITC Anti-Rat IgG
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