Product Information

Contents: Affinity Purified anti-human CD43 (Leukosialin, Sialophorin)
Catalog Number: 14-0439
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio84-3C1 (84-3C1)
Isotype: Mouse IgG1

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0439	Affinity Purified anti-human CD43 (Leukosialin, Sialophorin)	N/A	N/A	FC WB
11-0439	Fluorescein isothiocyanate (FITC) anti-human CD43 (Leukosialin, Sialophorin)	488	518	FC
12-0439	Phycoerythrin (PE) anti-human CD43 (Leukosialin, Sialophorin)	488	575	FC
17-0439	Allophycocyanin (APC) anti-human CD43 (Leukosialin, Sialophorin)	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio84-3C1 (84-3C1) antibody has been tested by flow cytometric analysis of human peripheral blood cells.

References

Borche L, Lozano F, Vilella R, Vives J. CD43 monoclonal antibodies recognize the large sialoglycoprotein of human leukocytes. Eur J Immunol. 1987 Oct;17(10):1523-6.

Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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