Product Information

Contents: Affinity Purified anti-human CD42b
Catalog Number: 14-0429
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: HIP1
Isotype: Mouse IgG1, κ
HLDA No.: IV P40

Surface staining of human platelets with anti-human CD42b (HIP1) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0429	Affinity Purified anti-human CD42b	N/A	N/A	FA FC IHC WB
16-0429	Functional Grade* Purified anti-human CD42b	N/A	N/A	FA FC
11-0429	FITC anti-human CD42b	488	518	FC
12-0429	PE anti-human CD42b	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIP1 antibody has been reported for use in flow cytometric analysis, in vitro blocking of human CD42b, immunoblotting (WB), and immunohistochemical staining. (Please use Functional Grade purified HIP1, cat. 16-0429, in functional assays.)

Applications Tested

The HIP1 antibody has been tested by flow cytometric analysis of human platelets and can be used less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibodies be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

George N., Q. Wei et al. Staphylcococcus aureus adhesion via Spa, ClfA, and SdrCDE to immobilized platelets demonstrates shear-dependent behavior. 2006. Arterioscler Thromb Vasc Biol 26:2394-2400. (FA, PubMed)

Gohda F., H. Uchiumi et al. 2007. Identification of inherited macrothrombocytopenias based on mean platelet volume among patients diagnosed with idiopathic thrombocytopenia. Thrombosis Research 119:741-746. (FC, PubMed)

Takahashi R., N. Sekine et al. 1999. Influence of Monoclonal Antiplatelet Glycoprotein Antibodies on In Vitro Human Megakaryocyte Colony Formation and Proplatelet Formation. Blood 93(6):1951-1958. (FA, PubMed)

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