human CD41a antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-human CD41
Catalog Number: 14-0419
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: HIP8
Isotype: Mouse IgG1, κ
HLDA No.: IV P38

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14-0419-80

25 ug

14-0419-82

100 ug

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Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0419	Affinity Purified anti-human CD41a	N/A	N/A	FC IHC
11-0419	FITC anti-human CD41a	488	518	FC
12-0419	PE anti-human CD41a	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIP8 monoclonal antibody reacts with the human CD41 molecule, the integrin α_IIb also known as platelet GPIIb. CD41 non-covalently associates with integrin β₃ (GPIIIa, CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin and mediates platelets aggregation. HIP8 blocks platelet aggregation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIP8 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested

The HIP8 antibody has been tested by flow cytometric analysis of human platelets. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-0418  FITC anti-human CD41b (clone HIP2)
Cat. 14-0418  Affinity Purified anti-human CD41b (clone HIP2)
Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control

data image 1

Staining of human platelets with purified mouse IgG1 isotype control (cat.#14-4714) (dotted histogram) or purified HIP8 (solid histogram) followed by FITC anti-mouse IgG (cat.#11-4011). Total viable cells were used for analysis.