Product Information

Contents: Affinity Purified anti-mouse CD41 (fibrinogen receptor, gpIIb/IIIa, integrin alpha IIb)
Catalog Number: 14-0411
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioMWReg30 (MWReg30)
Isotype: Rat IgG1, κ

Staining of mouse platelets with 0.125 μg of Purified Rat IgG1 Iso Cntrl (cat. 14-4301) (left) or 0.125 μg of Purified anti-mouse CD41 (MWReg30) (right) followed by PE Anti-Rat IgG (cat. 12-4822).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0411	Affinity Purified anti-mouse CD41 (fibrinogen receptor, gpIIb/IIIa, integrin alpha IIb)	N/A	N/A	ELISA FA FC IP
13-0411	Biotin anti-mouse CD41 (fibrinogen receptor, gpIIb/IIIa, integrin alpha IIb)	N/A	N/A	FC
11-0411	Fluorescein isothiocyanate (FITC) anti-mouse CD41 (fibrinogen receptor, gpIIb/IIIa, integrin alpha IIb)	488	518	FC
12-0411	Phycoerythrin (PE) anti-mouse CD41 (fibrinogen receptor, gpIIb/IIIa, integrin alpha IIb)	488	575	FC
17-0411	Allophycocyanin (APC) anti-mouse CD41 (fibrinogen receptor, gpIIb/IIIa, integrin alpha IIb)	633	660	FC
25-0411	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD41 (fibrinogen receptor, gpIIb/IIIa, integrin alpha IIb)	488	760	FC
48-0411	eFluor^TM 450 anti-mouse CD41 (fibrinogen receptor, gpIIb/IIIa, integrin alpha Iib)	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin αIIb). While initially thought to be expressed exclusively on the surface of platelets and megakaryocytes, it has been demonstrated that CD41 is also expressed on hematopoietic progenitors in the embryo, fetus and adult. CD41 associates with CD61 (gpIIIa, integrin βIII) to form a receptor which plays a major role in platelet function, including binding of several adhesion molecules such as fibrinogen, fibronectin and vitronectin.

Recently, the SLAM-family markers, CD48 and CD150 have been used to reliably identify hematopoietic stem cells (HSC). Specifically, it was found that CD150+CD48- bone marrow cells were highly efficient in their ability to confer long-term multi-lineage reconstitution in irradiated mice. Furthermore, the efficiency of reconstitution was enhanced when HSCs were further enriched through the exclusion of CD41+ cells. Thus, the use of CD150+CD48-CD41- as an expression profile efficiently identifies hematopoietic stem cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioMWReg30 (MWReg30) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and ELISA.

Applications Tested

This eBioMWReg30 (MWReg30) antibody has been tested by flow cytometric analysis of mouse platelets. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nieswandt B, Echtenacher B, Wachs FP, Schroder J, Gessner JE, Schmidt RE, Grau GE, Mannel DN. Acute systemic reaction and lung alterations induced by an antiplatelet integrin gpIIb/IIIa antibody in mice. Blood. 1999 Jul 15;94(2):684-93. (MWReg30, FC, FA, IHC, IP, PubMed)

Teeling JL, Jansen-Hendriks T, Kuijpers TW, de Haas M, van de Winkel JG, Hack CE, Bleeker WK. Therapeutic efficacy of intravenous immunoglobulin preparations depends on the immunoglobulin G dimers: studies in experimental immune thrombocytopenia. Blood. 2001 Aug 15;98(4):1095-9. (MWReg30, FA, PubMed)

Mitjavila-Garcia MT, Cailleret M, Godin I, Nogueira MM, Cohen-Solal K, Schiavon V, Lecluse Y, Le Pesteur F, Lagrue AH, Vainchenker W. Expression of CD41 on hematopoietic progenitors derived from embryonic hematopoietic cells. Development. 2002 Apr;129(8):2003-13. (MWReg30, FC, PubMed)

Bertrand JY, Giroux S, Golub R, Klaine M, Jalil A, Boucontet L, Godin I, Cumano A. Characterization of purified intraembryonic hematopoietic stem cells as a tool to define their site of origin. Proc Natl Acad Sci U S A. 2005 Jan 4;102(1):134-9. (MWReg30, FC, PubMed)

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