Product Information

Contents: Affinity Purified anti-human CD40
Catalog Number: 14-0409
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 5C3
Isotype: Mouse IgG1, κ
HLDA No.: V B-CD40.4

Surface staining of normal human peripheral blood cells with anti-human CD40 (5C3) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0409	Affinity Purified anti-human CD40	N/A	N/A	FA FC IH/F
16-0409	Functional Grade* Purified anti-human CD40	N/A	N/A	FA FC
11-0409	FITC anti-human CD40	488	518	FC
12-0409	PE anti-human CD40	488	575	FC
17-0409	Allophycocyanin (APC) anti-human CD40	633	660	FC
35-0409	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD40	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 5C3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. 5C3 has also been reported in in vitro functional studies. (Please use Functional Grade purified 5C3, cat. 16-0409, in functional assays.)

Applications Tested

The 5C3 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Pound JD, Challa A, Holder MJ, Armitage RJ, Dower SK, Fanslow WC, Kikutani H, Paulie S, Gregory CD, Gordon J. (1999). Minimal cross-linking and epitope requirements for CD40-dependent suppression of apoptosis contrast with those for promotion of the cell cycle and homotypic adhesions in human B cells. Int Immunol. Jan;11(1):11-20.

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