mouse CD40 antibody: purified antibody

Contents: Affinity Purified anti-mouse CD40
Catalog Number: 14-0402
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: HM40-3
Isotype: Armenian Hamster IgM, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-0402-81	50 ug	$65
14-0402-82	100 ug	$125
14-0402-85	500 ug	$220
14-0402-86	1 mg	$410

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes with 0.5 μg Purified Armenian Hamster IgG isotype control (cat. 16-4444) (open histogram) or 0.5 μg Purified anti-mouse CD40 (HM40-3) (colored histogram) followed by FITC anti-Armenian Hamster IgG (cat. 11-4111). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0402	FITC anti-mouse CD40	488	518	FC
13-0402	Biotin anti-mouse CD40	N/A	N/A	FC
14-0402	Affinity Purified anti-mouse CD40	N/A	N/A	FA FC IHC
16-0402	Functional Grade* Purified anti-mouse CD40	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HM40-3 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. HM40-3 has been reported in the literature to cross-react with the rat CD40 antigen.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HM40-3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. HM40-3 has also been reported in inhibition of binding of soluble CD154 to CD40 and in vitro activation of B cells. (Please use Functional Grade purified HM40-3, cat. 16-0402, in functional assays.)

Applications Tested

The HM40-3 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kashiwada, M., Y. Yagita, et al. 1996. Activation of mitogen-activated protein kinases via CD40 is distinct from that stimulated by surface IgM on B cells. Eur. J. Immunol. 26: 1451-58
Inaba, K., M. Witmer-Pack, et al. 1994. The tissue distrbution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J. Exp. Med. 180:1849-60.
Kawano, T., J. Cui, et al. 1997. CD1d-restricted and TCR-mediated activation of Va14 NKT cells by glycosylceramides. Science 278: 1626-9
Ferlin, W. G., E. Severinson, et al. 1996. CD40 signaling induces interleukin-4-independent IgE switching in vivo. Eur J Immunol 26(12): 2911-5.
Trinite, B., C. Voisine, et al. 2000. A subset of cytolytic dendritic cells in rat. J. Immunol. 165(8): 4202-8.

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