Product Information

Contents: Affinity Purified anti-human CD39 (ENTPD-1, NTPDase)
Catalog Number: 14-0399
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioA1 (A1)
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with 0.125 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or Purified anti-human CD39 (A1) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0399	Fluorescein isothiocyanate (FITC) anti-human CD39	488	518	FC
12-0399	Phycoerythrin (PE) anti-human CD39	488	575	FC
25-0399	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD39	488	760	FC
14-0399	Affinity Purified anti-human CD39	N/A	N/A	FC IH/F

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioA1 monoclonal antibody reacts with human CD39 also known as ENTPD1- ectonucleoside triphosphate diphosphohydrolase 1 or NTPDase. CD39 is an integral membrane protein with two transmembrane domains and exists as a homotetramer. It is the most prominent ectoenzyme of the immune system. The function of CD39 is to effectively remove toxic extracellular ATP by converting it to ADP or AMP. CD39 is thought to work together with CD73 to hydrolyze ATP and has been well characterized on langerhan cells. Expression of CD39 is originally identified on activated lymphocytes. Expression is also found on a subset of T cells, B cells and dendritic cells with weak staining on monocytes and granulocytes.
Recently CD39 and CD73 have been found on regulatory T cells specifically the effector/memory like T cells. Functionally CD39 may allow Tregs to enter inflamed areas where high levels of ATP are present. Expression of Foxp3+ Cd4 positives cells range from 25-45% CD25+/CD39+ which his consistent with the findings of Borsellino et al.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioA1 (A1) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested

This eBioA1 (A1) antibody has been tested by flow cytometric analysis of nomral human peripheral blood cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Aversa GG., M.G. Suranyi, J.A. Waugh, A.G. Bishop, B.M. Hall. 1988. Detection of a Late Lymphocyte Activation Marker by A1, a New Monoclonal Antibody. Transplant Proc. 20(1):49-52.

Aversa GG., J.A. Waugh, G.A. Bishop, B.M. Hall. 1989. Use of Monoclonal Antibodies to Study in vivo and in vitro-activated Lymphocytes. Transplant Proc. 21(1):349-50.

Stockl J., O. Majdic, G. Fischer, D. Maurer, W. Knapp. 2001. Monomorphic Molecules Function as Additional Recognition Structures on Haptenated Target Cells for HLA-A1-Restricted, Hapten-Specific CTL. J. Immunol. 167L:2724-2733

Borsellino G, Kleinewietfeld M, Di Mitri D, Sternjak A, Diamantini A, Giometto R, Hopner S, Centonze D, Bernardi G, Dell'acqua ML, Rossini PM, Battistini L, Rotzschke O, Falk K. Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression. Blood. 2007 Apr 20; [Epub ahead of print]

Lyck L, Dalmau I, et al. 2008. Immunohistochemical markers for quantitative studies of neurons and glia in human neocortex. J Histochem Cytochem. 56(3):201-21. (A1, IHC frozen, PubMed)

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Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control