Product Information

Contents: Purified anti-human CD36
Catalog Number: 14-0369
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioNL07 (NL07)
Isotype: Mouse IgM

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgM Iso Cntrl (cat. 14-4752) (open histogram) or 0.25 μg of Purified anti-human CD36 (NL07) (colored histogram) followed by PE anti-mouse IgM (cat. 12-5890). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0369	Fluorescein isothiocyanate (FITC) anti-human CD36	488	518	FC
14-0369	Purified anti-human CD36	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody eBioNL07 recognizes human CD36, which is a member of the class B scavenger receptor family. CD36 was originally identified as a platelet-membrane glycoprotein also called glycoprotein IV and a receptor for thrombospondin-1 (TSP-1) and extracellular matrix proteins. Binding to TSP-1 is in the CLESH (CD36 LIMP-II Emp sequence homology) domain of CD36. CD36 expression is broad and includes microvascular (but not large vessel) endothelium, adipocytes, skeletal muscle, dendritic cells, epithelia of the retina, breast, and intestine, smooth muscle cells, and hematopoietic cells, including erythroid precursors, platelets, monocytes/macrophages, DCs and megakaryocytes. Expression on platelets is absent on Nak^a negative donors. Unlike other scavenger receptor, CD36 binds LDL that has been exposed to “minimally” oxidizing conditions. CD36 is also a fatty acid translocase (FAT) necessary for the transport of long-chain fatty acids (LCFAs) and therefore may play a role in atherosclerosis.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioNL07 (NL07) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioNL07 (NL07) antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Alessio M, Greco NJ, Primo L, Ghigo D, Bosia A, Tandon NN, Ockenhouse CF, Jamieson GA, Malavasi F. Platelet activation and inhibition of malarial cytoadherence by the anti-CD36 IgM monoclonal antibody NL07. Blood. 1993 Dec 15;82(12):3637-47. (NL07, FC, PubMed)

Alessio M, Ghigo D, Garbarino G, Geuna M, Malavasi F. Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07. Cell Immunol. 1991 Oct 15;137(2):487-500. (NL07, FC, PubMed)

Gruarin P, Ulliers D, Thorne RF, Alessio M. Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb. Mol Cell Biochem. 2000 Nov;214(1-2):89-95. (NL07, PubMed)

Related Products

Cat. 14-0361    Purified anti-mouse CD36 (clone No.72-1)
Cat. 14-4752    Affinity Purified Mouse IgM Isotype Control
Cat. 12-5890    PE anti-mouse IgM (clone eB121-15F9)