Product Information

Contents: Affinity Purified anti-mouse CD36
Catalog Number: 14-0361
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: No.72-1
Isotype: Rat IgG2a, κ

Staining of J774.1 cells with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified anti-mouse CD36 (No.72-1) (colored histogram) followed by PE Anti-Rat IgG (cat. 12-4822). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0361	Purified anti-mouse CD36	N/A	N/A	FC
12-0361	Phycoerythrin (PE) anti-mouse CD36	488	575	FC
51-0361	Alexa Fluor® 647 anti-mouse CD36	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The No. 72-1 monoclonal antibody reacts with mouse CD36, which is a 88 kDa member of the class B scavenger receptor family. Murine CD36 was discovered using an expression cloning strategy to identify macrophage receptors that recognized oxidized low-density lipoprotein (oxLDL), but not acetylated low-density lipoprotein (LDL). CD36 is a platelet-membrane glycoprotein, but is also expressed on monocytes/macrophages, granulocytes, B lymphocytes, microvascular endothelial cells, adipocytes, skeletal muscle and dendritic cells. The role of CD36 as a scavenger receptor was confirmed by the CD36-deficient mouse, which was defective in the binding and uptake of oxLDL by peritoneal macrophages. In addition to binding oxLDL, CD36 binds other ligands including TSP-1, anionic phospholipids, apoptotic cells, collagen, Plasmodium falciparum-infected erythrocytes and long-chain fatty acids.

Testing also shows staining on some B cells in the bone marrow in addition to the myeloid cells. For more information, please contact tech@ebioscience.com .

Applications Reported

For research use only, not for diagnostic or therapeutic use. This No.72-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This No.72-1 antibody has been tested by flow cytometric analysis of J7441.1 cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Direct conjugates are recommended for flow cytometric studies.

References

Endemann G, Stanton LW, Madden KS, Bryant CM, White RT, Protter AA. CD36 is a receptor for oxidized low density lipoprotein. J Biol Chem. 1993 Jun 5;268(16):11811-6.

Febbraio M, Podrez EA, Smith JD, Hajjar DP, Hazen SL, Hoff HF, Sharma K, Silverstein RL. Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice. J Clin Invest. 2000 Apr;105(8):1049-56.

Zhang P, Li W, Wang Y, Hou L, Xing Y, Qin H, Wang J, Liang Y, Han H. Identification of CD36 as a new surface marker of marginal zone B cells by transcriptomic analysis. Mol Immunol. 2007 Jan;44(4):332-7.

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