rat Pan endothelium antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-rat Pan endothelium
Catalog Number: 14-0360
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: HIS52
Isotype: Mouse IgG1, κ

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14-0360-82

100 ug

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0360	Affinity Purified anti-rat Pan endothelium	N/A	N/A	FC IH/F
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIS52 monoclonal antibody reacts with a molecule expressed on the surface of all rat endothelial cells, also known as Rat Endothelium Cell Antigen-1 (RECA-1). The exact nature of HIS52 antigen is, however, not known. HIS52 does not cross react with various species tested other than rat. This antibody is a very useful tool as a specific phenotypic marker for rat endothelium.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIS52 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. No reactivity of the antibody was seen when applied in immunoblotting of PAGE-run lysates from endothelial cell cultures and stromal cell preparations.

Applications Tested

The HIS52 antibody has been tested by immunohistochemistry of rat frozen sections and can be used at 1-10 μg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ricono JM, Xu YC, Arar M, Jin DC, Barnes JL, Abboud HE. 2003. Morphological insights into the origin of glomerular endothelial and mesangial cells and their precursors.
J Histochem Cytochem. 51(2):141-50.
Duijvestijn AM, van Goor H, Klatter F, Majoor GD, van Bussel E, van Breda Vriesman PJ. 1992. Antibodies defining rat endothelial cells: RECA-1, a pan-endothelial cell-specific monoclonal antibody. Lab Invest. 66(4):459-66.

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