Product Information

Contents: Affinity Purified anti-human CD34 - reacts with Class III epitope on CD34 (Mucosialin)
Catalog Number: 14-0349
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 4H11
Isotype: Mouse IgG1

Staining of TF-1 cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human CD34 (4H11) (colored histogram) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (cat. 12-4012). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0349	Phycoerythrin (PE) anti-human CD34 - reacts with Class III epitope on CD34 (Mucosialin)	488	575	FC
25-0349	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD34 - reacts with Class III epitope on CD34 (Mucosialin)	488	760	FC
51-0349	Alexa Fluor® 647 anti-human CD34 - reacts with Class III epitope on CD34 (Mucosialin)	633	668	FC
14-0349	Affinity Purified anti-human CD34 - reacts with Class III epitope on CD34 (Mucosialin)	N/A	N/A	FA FC WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of this family are single pass transmembrane proteins with a heavily glycosylated, extracellular, and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines and also by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage with neuraminidase, chymopapain and O-glycoprotease. According to this analysis, the 4H11 antibody belongs to class III, indicating that it reacts with a protein epitope.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 4H11 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB). The 4H11 monoclonal antibody can also be used to inhibit proliferation and induce apoptosis in CD34+ cell lines including MOLM-9, JURL-MK1 and HEL.

Applications Tested

This 4H11 antibody has been tested by flow cytometric analysis of TF-1 cells. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Elknerová K, Lacinová Z, Soucek J, Marinov I, Stöckbauer P. Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines. Neoplasma. 2007;54(4):311-20. (4H11, FA, FC, PubMed)

Sutherland DR, Watt SM, Dowden G, Karhi K, Baker MA, Greaves MF, Smart JE. Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34. Leukemia. 1988 Dec;2(12):793-803.

Baumheter S, Singer MS, Henzel W, Hemmerich S, Renz M, Rosen SD, Lasky LA. Binding of L-selectin to the vascular sialomucin CD34. Science. 1993 Oct 15;262(5132):436-8.

Related Products

Cat. 14-0341    Affinity Purified anti-mouse CD34 (clone RAM34)
Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control