Print Page

Adobe PDF

Human Cellular Antigens Chart

Contents: Affinity Purified anti-human CD33 Catalog Number: 14-0339 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: HIM3-4 Isotype: Mouse IgG1, κ HLDA No.: V MA112 Prices for This Product* Cat. No. Size Price Qty Action 14-0339-80 25 ug $50 14-0339-82 100 ug $95 *International customers: Please contact your distributor for region specific pricing.

Surface staining of normal human peripheral blood cells with anti-human CD33 (HIM3-4) PE. Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0339	FITC anti-human CD33	488	518	FC
12-0339	PE anti-human CD33	488	575	FC
14-0339	Affinity Purified anti-human CD33	N/A	N/A	FC
15-0339	PE-Cy5 anti-human CD33	488	670	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

---

Description

---

The HIM3-4 monoclonal antibody reacts with human CD33, a 67 kDa member of the sialoadhesion family. CD33 is expressed by myelomonocytic precursors, monocytes, mast cells, and granulocytes. Hematopoietic stem cells and lymphocytes do not express this antigen. CD33 plays a role in sialic-acid dependent cell adhesion.

---

Applications Reported

---

For research use only, not for diagnostic or therapeutic use. The HIM3-4 antibody has been reported for use in flow cytometric analysis.

---

Applications Tested

---

The HIM3-4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

---

References

---

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

---

Related Products

---

Cat. 11-0338    FITC anti-human CD33 (clone WM-53 (WM53))
Cat. 12-0338    PE anti-human CD33 (clone WM-53 (WM53))
Cat. 14-0338    Affinity Purified anti-human CD33 (clone WM-53 (WM53))
Cat. 15-0338    Phycoerythrin-Cy5 (PE-Cy5) anti-human CD33 (clone WM-53 (WM53))
Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control