Product Information

Contents: Affinity Purified anti-human CD33
Catalog Number: 14-0338
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: WM-53 (WM53)
Isotype: Mouse IgG1, κ
HLDA No.: VI M505

Staining of normal human peripheral blood cells with 0.5 µg of purified mouse IgG1 isotype control (cat.14-4714) (open histogram) or 0.5 µg of purified WM-53 (colored histogram) followed by FITC anti-mouse IgG (cat.11-4011). Cells in the monocyte (left) and granulocyte (right) gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0338	FITC anti-human CD33	488	518	FC
12-0338	PE anti-human CD33	488	575	FC
14-0338	Affinity Purified anti-human CD33	N/A	N/A	FC IHC IP MIC
15-0338	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD33	488	670	FC
25-0338	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD33	488	760	FC
45-0338	PerCP-Cy5.5 anti-human CD33	488	690	FC
56-0338	Alexa Fluor® 700 anti-human CD33 (APC-Cy5.5 Equivalent)	633	723	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The WM-53 (WM53) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, microscopy, and immunohistochemical staining.

Applications Tested

The WM-53 (WM53) antibody has been tested by flow cytometric analysis of lysed whole blood. This can be used at less than or equal to 1 µg per million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC. 1987 Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33). Dis Markers. 5(4):215-25.

Related Products

Cat. 11-0339    FITC anti-human CD33 (clone HIM3-4)
Cat. 12-0339    PE anti-human CD33 (clone HIM3-4)
Cat. 14-0339    Affinity Purified anti-human CD33 (clone HIM3-4)
Cat. 15-0339    PE-Cy5 anti-human CD33 (clone HIM3-4)
Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control