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Human Cellular Antigens Chart

Contents: Affinity Purified anti-human CD33 Catalog Number: 14-0338 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: WM-53 (WM53) Isotype: Mouse IgG1, κ HLDA No.: VI M505 Prices for This Product* Cat. No. Size Price Qty Action 14-0338-80 25 ug $50 14-0338-82 100 ug $95 *International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with 0.5 µg of purified mouse IgG1 isotype control (cat.14-4714) (open histogram) or 0.5 µg of purified WM-53 (colored histogram) followed by FITC anti-mouse IgG (cat.11-4011). Cells in the monocyte (left) and granulocyte (right) gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0338	FITC anti-human CD33	488	518	FC
12-0338	PE anti-human CD33	488	575	FC
14-0338	Affinity Purified anti-human CD33	N/A	N/A	FC  IHC  IP  MIC
15-0338	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD33	488	670	FC
25-0338	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD33	488	760	FC
45-0338	PerCP-Cy5.5 anti-human CD33	488	690	FC
56-0338	Alexa Fluor® 700 anti-human CD33 (APC-Cy5.5 Equivalent)	633	723	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The WM-53 (WM53) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, microscopy, and immunohistochemical staining.

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Applications Tested

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The WM-53 (WM53) antibody has been tested by flow cytometric analysis of lysed whole blood. This can be used at less than or equal to 1 µg per million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC. 1987 Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33). Dis Markers. 5(4):215-25.

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Related Products

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Cat. 11-0339    FITC anti-human CD33 (clone HIM3-4)
Cat. 12-0339    PE anti-human CD33 (clone HIM3-4)
Cat. 14-0339    Affinity Purified anti-human CD33 (clone HIM3-4)
Cat. 15-0339    PE-Cy5 anti-human CD33 (clone HIM3-4)
Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control