anti-mouse CD31 antibody: purified anti-mouse PECAM-1 antibody

Contents: Affinity Purified anti-mouse CD31 (PECAM-1)
Catalog Number: 14-0311
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 390
Isotype: Rat IgG2a, κ

Product Options		Price
Cat. No.	Size	To view relevant pricing, please select your country and resume browsing.
14-0311-81	50 ug
14-0311-82	100 ug
14-0311-85	500 ug

Surface staining of mouse splenocytes with anti-mouse CD31 (390) FITC (left), and PE (right). Autofluorescence is shown via open histogram. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0311	Affinity Purified anti-mouse CD31 (PECAM-1)	N/A	N/A	FC IH/F IP
16-0311	Functional Grade* Purified anti-mouse CD31 (PECAM-1)	N/A	N/A	FA FC
13-0311	Biotin anti-mouse CD31 (PECAM-1)	N/A	N/A	FC
11-0311	FITC anti-mouse CD31 (PECAM-1)	488	518	FC
12-0311	PE anti-mouse CD31 (PECAM-1)	488	575	FC
17-0311	Allophycocyanin (APC) anti-mouse CD31 (PECAM-1)	633	660	FC
25-0311	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD31 (PECAM-1)	488	760	FC
36-0311	Functional Grade* Biotin anti-mouse CD31 (PECAM-1)	N/A	N/A	FC
93-0311	eFluor^TM 605^NC anti-mouse CD31 (PECAM-1)	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 390 monoclonal antibody reacts with mouse CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on all leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 390 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. 390 has also been reported in in vitro functional studies. (Please use Functional Grade purified 390, cat. 16-0311, in functional assays.)

Applications Tested

The 390 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sadri N, Schneider RJ. Auf1/Hnrnpd-Deficient Mice Develop Pruritic Inflammatory Skin Disease. J Invest Dermatol. 2008 Oct 2. [Epub ahead of print]. (390, IHC frozen, PubMed)

Cao G, O'Brien CD, Zhou Z, Sanders SM, Greenbaum JN, Makrigiannakis A, DeLisser HM. Involvement of human PECAM-1 in angiogenesis and in vitro endothelial cell migration. Am J Physiol Cell Physiol. 2002 May;282(5):C1181-90. (390, IHC frozen)

Wiewrodt R, Anu P. Thomas, Luca Cipelletti, Melpo Christofidou-Solomidou, David A. Weitz, Sheldon I. Feinstein, David Schaffer, Steven M. Albelda, Michael Koval, and Vladimir R. Muzykantov. Size-dependent intracellular immunotargeting of therapeutic cargoes into endothelial cells.Blood. 2002 Feb 1;99(3):912-22.

Horenstein AL, Stockinger H, Imhof BA, Malavasi F. CD38 binding to human myeloid cells is mediated by mouse and human CD31. Biochem J. 1998 Mar 15;330 ( Pt 3):1129-35.

Baldwin HS, Shen HM, Yan HC, DeLisser HM, Chung A, Mickanin C, Trask T, Kirschbaum NE, Newman PJ, Albelda SM, et al. Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development. Development. 1994 Sep;120(9):2539-53.

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