Product Information

Contents: Affinity Purified anti-human CD29 (Integrin β₁)
Catalog Number: 14-0297
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MEM-101A
Isotype: Mouse IgG1
HLDA No.: VI

Surface staining of normal human peripheral blood cells with anti-human CD29 (MEM-101A) PE (left), and PE-Cy5 (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
15-0297	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD29 (Integrin beta 1)	488	670	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MEM-101A monoclonal antibody reacts with human CD29, an approximately 130 kDa integrin β₁. CD29 complexes with one of nine integrin α subunits to form the very late antigen (VLA) subfamily of adhesion molecules. CD29 is expressed broadly on lymphocytes, monocytes and granulocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MEM-101A antibody has been reported for use in flow cytometric analysis.

Applications Tested

The MEM-101A antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kishimoto, T., A.E.G., von dem Borne, et al. eds. 1998. Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.

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