Product Information

Contents: Affinity Purified anti-mouse CD29 (Integrin β1 chain, α4β1, VLA-4)
Catalog Number: 14-0292
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: KMI6
Isotype: Rat IgG2a, κ

Balb/c spleen cell lysate (3x10⁵ cells/lane) was resolved by SDS-PAGE under non-reducing conditions, immunoblotted with 2 µg/mL Affinity Purified anti-mouse CD29 (clone KMI6), and revealed with HRP-conjugated anti-rat IgG.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0292	Affinity Purified anti-mouse CD29 (Integrin b1 chain, CD49d, a4b1, VLA-4)	N/A	N/A	FA FC IH/F IP WB
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The KMI6 monoclonal antibody reacts with mouse CD29 (integrin β1), a 120-130 kDa member of the β integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the α integrins CD49a-f, to form the VLA-1 through VLA-6 complexes, respectively, and also with CD51. These αβ integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All of the integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins also interact with cellular receptors including VCAM-1 and MadCAM-1. The KMI6 antibody has been shown to be an activating antibody for β1 integrins, enhancing lymphocyte binding to fibronectin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This KMI6 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. The KMI6 antibody has also been found useful for immunoblotting, recognizing a protein of approximately 120 kD under non-reducing conditions.

Applications Tested

This KMI6 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. The KMI6 antibody has also been tested by immunoblotting at 2 μg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Aoyama K, Oritani K, Yokota T, Ishikawa J, Nishiura T, Miyake K, Kanakura Y, Tomiyama Y, Kincade PW, Matsuzawa Y. Stromal cell CD9 regulates differentiation of hematopoietic stem/progenitor cells. Blood. 1999 Apr 15;93(8):2586-94. (KMI6, IP, WB, PubMed)

Wadsworth SA, Chang AC, Hong MJ, Halvorson MJ, Otto S, Coligan JE. Expression of a novel integrin β1 chain epitope and anti-β1 antibody-mediated enhancement of fibronectin binding are dependent on the stage of T cell differentiation. J Immunol. 1995 Mar 1;154(5):2125-33. (KMI6, FC, IP, FA, PubMed)

Jacobsen K, Miyake K, Kincade PW, Osmond DG. Highly restricted expression of a stromal cell determinant in mouse bone marrow in vivo. J Exp Med. 1992 Oct 1;176(4):927-35. (KMI6, IP, PubMed)

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