Product Information

Contents: Affinity Purified anti-mouse CD29 (Integrin beta 1)
Catalog Number: 14-0291
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioHMb1-1 (HMb1-1)
Isotype: Armenian Hamster IgG

Staining of C57Bl/6 thymocytes with 0.5 μg of Purified Armenian Hamster IgG Iso Cntrl (cat. 14-4888) (open histogram) or 0.5 μg of Purified anti-mouse CD29 (HMb1-1) (colored histogram) followed by Biotin Anti-Armenian Hamster IgG (cat. 13-4113)and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0291	Affinity Purified anti-mouse CD29 (Integrin \b1 Chain)	N/A	N/A	FA FC IP
16-0291	Functional Grade* Purified anti-mouse CD29 (Integrin \b1 Chain)	N/A	N/A	FA FC
12-0291	Phycoerythrin (PE) anti-mouse CD29 anti-mouse CD29 (Integrin \b1 Chain)	488	575	FC
17-0291	Allophycocyanin (APC) anti-mouse CD29 (Integrin \b1 Chain)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioHMb1-1 monoclonal antibody reacts with mouse CD29 (integrin β₁), a 110-120 kDa member of the β integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the α integrins CD49a-f, to form the VLA-1 through VLA-6 complexes, respectively, and also with CD51. These αβ integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All of the integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins also interact with cellular receptors including VCAM-1 and MadCAM-1.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioHMb1-1 (HMb1-1) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. (Please use Functional Grade purified eBioHMb1-1 (HMb1-1), cat. 16-0291, in functional assays.)

Applications Tested

This eBioHMb1-1 (HMb1-1) antibody has been tested by flow cytometric analysis of mouse spleen, thymus and bone marrow cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Noto K, Kato K, Okumura K, Yagita H. Identification and functional characterization of mouse CD29 with a mAb. Int Immunol. 1995 May;7(5):835-42. (HMβ1-1, mAb development, IP, FA, PubMed)

Ridger VC, Wagner BE, Wallace WA, Hellewell PG. Differential effects of CD18, CD29, and CD49 integrin subunit inhibition on neutrophil migration in pulmonary inflammation. J Immunol. 2001 Mar 1;166(5):3484-90. (HMβ1-1, FA, PubMed)

Related Products

Cat. 14-0297    Purified anti-human CD29 (Integrin beta 1) (clone MEM-101A)
Cat. 11-4111    FITC Anti-Armenian Hamster IgG
Cat. 13-4113    Biotin Anti-Armenian Hamster IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4888    Affinity Purified Armenian Hamster IgG Isotype Control (clone eBio299Arm)