mouse CD28 antibody: purified antibody

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Contents: Affinity Purified anti-mouse CD28
Catalog Number: 14-0281
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 37.51
Isotype: Golden Syrian Hamster IgG

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-0281-81	50 ug	$55
14-0281-82	100 ug	$85
14-0281-85	500 ug	$220
14-0281-86	1 mg	$410

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c thymocytes with 0.25 μg of Purified Golden Syrian Hamster IgG Iso Cntrl (cat. 14-4914) (open histogram) or 0.25 μg of Purified anti-mouse CD28 (37.51) (colored histogram) followed by FITC Anti-Syrian Hamster IgG (cat. 11-4211). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0281	PE anti-mouse CD28	488	575	FC
13-0281	Biotin anti-mouse CD28	N/A	N/A	FC
14-0281	Affinity Purified anti-mouse CD28	N/A	N/A	FA FC IHC IP
15-0281	PE-Cy5 anti-mouse CD28	488	670	FC
16-0281	Functional Grade* Purified anti-mouse CD28	N/A	N/A	FA FC
17-0281	APC anti-mouse CD28	633	660	FC
25-0281	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD28	488	760	FC
45-0281	PerCP-Cy5.5 anti-mouse CD28	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 37.51 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 37.51 has also been reported in costimulation of T cells in vitro and in vivo. (Please use Functional Grade purified 37.51, cat. 16-0281, in functional assays.)

Applications Tested

The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained.

References

Nandi, D., J. A. Gross, et al. (1994). "CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells." J Immunol 152(7): 3361-9.
Gross, J. A., E. Callas, et al. (1992). "Identification and distribution of the costimulatory receptor CD28 in the mouse." J Immunol 149(2): 380-8.
Harding, F. A., J. G. McArthur, et al. (1992). "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." Nature 356(6370): 607-9.
Gross, J. A., T. St. John, et al. (1990). "The murine homologue of the T lymphocyte antigen CD28. Molecular cloning and cell surface expression." J Immunol 144(8): 3201-10.

Related Products

Cat. 11-4211    FITC Anti-Syrian Hamster IgG (clone Polyclonal)
Cat. 13-4213    Biotin Anti-Syrian Hamster IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4914    Affinity Purified Golden Syrian Hamster IgG Isotype Control (clone n/a)