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Human Cellular Antigens Chart

Contents: Affinity Purified anti-human CD27 Catalog Number: 14-0279 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: O323 Isotype: Mouse IgG1, κ HLDA No.: IV T-186 Prices for This Product* Cat. No. Size Price Qty Action 14-0279-80 25 ug $50 14-0279-82 100 ug $95 *International customers: Please contact your distributor for region specific pricing.

Surface staining of normal human peripheral blood cells with anti-human CD27 (O323) PE (left), and APC (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
10-0279	**May be discontinued; see superior product APC-Alexa Fluor® 750** APC-Cy7 anti-human CD27	633	760	FC
11-0279	FITC anti-human CD27	488	518	FC
12-0279	PE anti-human CD27	488	575	FC
13-0279	Biotin anti-human CD27	N/A	N/A	FC
14-0279	Affinity Purified anti-human CD27	N/A	N/A	FC
15-0279	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD27	488	670	FC
17-0279	APC anti-human CD27	633	660	FC
25-0279	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD27	488	760	FC
27-0279	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD27 (APC-Cy7 Equivalent)	633	779	FC
56-0279	Alexa Fluor® 700 anti-human CD27 (APC-Cy5.5 Equivalent)	633	723	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The O323 antibody has been reported for use in flow cytometric analysis.

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Applications Tested

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The O323 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

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Related Products

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Cat. 11-0271    FITC anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 12-0271    PE anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 13-0271    Biotin anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 14-0271    Affinity Purified anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 16-0271    Functional Grade Purified anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 17-0271    APC anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control