Product Information

Contents: Affinity Purified anti-human CD25 (interleukin-2 receptor, IL-2R)
Catalog Number: 14-0259
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: BC96
Isotype: Mouse IgG1, κ
HLDA No.: V T-072

Surface staining of PHA stimulated human PBMC with PE anti-human CD25 (BC96). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0259	May be discontinued; see superior product Alexa Fluor® 488 FITC anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	518	FC
12-0259	PE anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	575	FC
13-0259	Biotin anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	N/A	N/A	FC
14-0259	Affinity Purified anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	N/A	N/A	FC
15-0259	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	670	FC
17-0259	Allophycocyanin (APC) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	633	660	FC
25-0259	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	760	FC
45-0259	PerCP-Cy5.5 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	690	FC
53-0259	Alexa Fluor® 488 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	519	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor β chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The BC96 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The BC96 antibody has been tested by flow cytometric analysis of PHA-activated human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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