Product Information

Contents: Affinity Purified anti-human CD23 (Low affinity IgE receptor)
Catalog Number: 14-0239
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.1% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: Tu1
Isotype: Mouse IgG3, κ
HLDA No.: I B20

Description

The Tu1 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets. Expression of CD23 is upregulated upon B cell activation, and soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation. CD21, and the α subunit of CD11b and CD11c bind to CD23.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The Tu1 antibody has been reported for use in flow cytometric analysis, immunohistochemistry of frozen tissues, and immunoprecipitation.

Applications Tested

The Tu1 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes and can be used at less than or equal to 1 µg per 100 µl blood. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Bernard, A., et al. eds. (1981). Leukocyte Typing. Springer-Verlag.

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