Product Information

Contents: Affinity Purified anti-human CD23 (FcεRII)
Catalog Number: 14-0238
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: EBVCS2
Isotype: Mouse IgG1
HLDA No.: N/A

Staining of normal human peripheral blood cells with 0.5 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified EBVCS2 (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0238	Fluorescein (FITC) anti-human CD23 (FceRII)	488	518	FC IH/F
12-0238	Phycoerythrin (PE) anti-human CD23 (FceRII)	488	575	FC
17-0238	Allophycocyanin (APC) anti-human CD23 (FceRII)	633	660	FC
25-0238	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD23 (FceRII)	488	760	FC
14-0238	Affinity Purified anti-human CD23 (FceRII)	N/A	N/A	FC IH/F

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The EBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets. Expression of CD23 is upregulated upon B cell activation, and soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation. CD21, and the α subunit of CD11b and CD11c bind to CD23.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The EBVCS2 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The EBVCS2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Bernard, A., et al. eds. (1981). Leukocyte Typing. Springer-Verlag.

Related Products

Cat. 11-0239    FITC anti-human CD23 (FceRII) (clone Tu1)
Cat. 12-0239    PE anti-human CD23 (FceRII) (clone Tu1)
Cat. 14-0239    Affinity Purified anti-human CD23 (FceRII) (clone Tu1)
Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control