Product Information

Contents: Affinity Purified anti-human CD22
Catalog Number: 14-0229
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio4KB128 (4KB128)
Isotype: Mouse IgG1, κ

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human CD22 (4KB128) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0229	Affinity Purified anti-human CD22	N/A	N/A	FC IHC
11-0229	Fluorescein isothiocyanate (FITC) anti-human CD22	488	518	FC
12-0229	Phycoerythrin (PE) anti-human CD22	488	575	FC
15-0229	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD22	488	670	FC
35-0229	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD22	488	690	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2), which is a member of the siglec subgroup of the Ig superfamily. CD22 is a type I transmembrane glycoprotein composed of two polypeptide chains, CD22α and CD22β, of 130 and 140 kDa respectively, produced by alternative splicing of the CD22 gene. CD22 is expressed at high levels on mature B cells and B cell lymphomas. The extracellular portion of CD22 contains seven Ig-like domains, some of which are capable of binding ligands with sialic acid moieties expressed on epithelial, endothelial, B and T cells. The intracellular portion of CD22 contains 6 tyrosine residues contained within immunotyrosine-based inhibitory motifs (ITIM) and immunotyrosine-based activation-like motifs, which are phosphorylated upon B-cell receptor engagement, which enables CD22 to participate in the positive and negative regulation of B-cell receptor signaling.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio4KB128 (4KB128) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested

This eBio4KB128 (4KB128) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Mason DY, Stein H, Gerdes J, Pulford KA, Ralfkiaer E, Falini B, Erber WN, Micklem K, Gatter KC. Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid cells. Blood. 1987 Mar;69(3):836-40. (4KB128, IHC, PubMed)

Campana D, Janossy G, Bofill M, Trejdosiewicz LK, Ma D, Hoffbrand AV, Mason DY, Lebacq AM, Forster HK. Human B cell development. I. Phenotypic differences of B lymphocytes in the bone marrow and peripheral lymphoid tissue. J Immunol. 1985 Mar;134(3):1524-30.

Nitschke L. The role of CD22 and other inhibitory co-receptors in B-cell activation. Curr Opin Immunol. 2005 Jun;17(3):290-7. Review.

Related Products

Cat. 14-0221  Affinity Purified anti-mouse CD22 (clone 2D6)
Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control