Product Information

Contents: Affinity Purified anti-mouse CD21/CD35 (CR2/CR1)
Catalog Number: 14-0212
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio4E3 (4E3)
Isotype: Rat IgG2a, λ

Staining of BALB/c splenocytes with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified anti-mouse CD21/CD35 (eBio4E3) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0212	Affinity Purified anti-mouse CD21/CD35 (CR2/CR1)	N/A	N/A	FC
16-0212	Functional Grade* Purified anti-mouse CD21/CD35 (CR2/CR1)	N/A	N/A	FA FC
11-0212	Fluorescein isothiocyanate (FITC) anti-mouse CD21/CD35 (CR2/CR1)	488	518	FC
57-0212	Pacific Blue® anti-mouse CD21/CD35 (CR2/CR1)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody eBio4E3 reacts with an eptiope shared by mouse CD21 (CR2) and CD35 (CR1). CD21 and CD35 are alternatively spliced transcripts from the Cr2 gene, which result in cell-surface proteins of 145 and 190 kDa, respectively. CD21 and CD35 are expressed by mature B cells, but not on thymocytes, peripheral T cells, erythrocytes or platelets. Furthermore, there is little evidence which demonstrates their expression on macrophages. CD21 is a receptor for complement component C3d and Epstein-Barr virus (EBV), and in association with CD19 and CD81, CD21 also participates in B-cell activation through the B cell receptor. Cr2 deficient mice display impaired inflammatory and humoral immune responses in vivo. The anti-mouse CD21/35 monoclonal antibody clones eBio4E3 and eBio8D9 do not cross-block each other suggesting that they bind to different epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio4E3 (4E3) antibody has been reported for use in flow cytometric analysis. Please use Functional Grade eBio4E3 (cat. 16-0212) in functional assays.

Applications Tested

This eBio4E3 (4E3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kozono Y, Abe R, Kozono H, Kelly RG, Azuma T, and Holers VM. 1998. Cross-linking CD21/CD35 or CD19 increases both B7-1 and B7-2 expression on murine splenic B cells. Journal of Immunology. 160: 1565-1572. (8D9, FA, PubMed)

Martin, B.K., and J.H. Weis. 1993. Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products. Eur. J. Immunol. 23: 3037-3042.

Related Products

Cat. 11-0211  Fluorescein isothiocyanate (FITC) anti-mouse CD21/CD35 (CR2/CR1) (clone eBio8D9 (8D9))
Cat. 12-0211  Phycoerythrin (PE) anti-mouse CD21/CD35 (CR2/CR1) (clone eBio8D9 (8D9))
Cat. 13-0211  Biotin anti-mouse CD21/CD35 (CR2/CR1) (clone eBio8D9 (8D9))
Cat. 14-0211  Affinity Purified anti-mouse CD21/CD35 (CR2/CR1) (clone eBio8D9 (8D9))
Cat. 16-0211  Functional Grade Purified anti-mouse CD21/CD35 (CR2/CR1) (clone eBio8D9 (8D9))
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4321  Affinity Purified Rat IgG2a Isotype Control
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)