Product Information

Contents: Affinity Purified anti-mouse CD21/CD35 (CR2/CR1)
Catalog Number: 14-0211
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio8D9 (8D9)
Isotype: Rat IgG2a, λ

Staining of C57Bl/6 splenocytes with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified anti-mouse CD21/CD35 (eBio8D9) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0211	Affinity Purified anti-mouse CD21/CD35 (CR2/CR1)	N/A	N/A	FA FC WB
16-0211	Functional Grade* Purified anti-mouse CD21/CD35 (CR2/CR1)	N/A	N/A	FA FC
13-0211	Biotin anti-mouse CD21/CD35 (CR2/CR1)	N/A	N/A	FC
11-0211	Fluorescein isothiocyanate (FITC) anti-mouse CD21/CD35 (CR2/CR1)	488	518	FC
12-0211	Phycoerythrin (PE) anti-mouse CD21/CD35 (CR2/CR1)	488	575	FC
25-0211	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD21/CD35 (CR2/CR1)	488	760	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody eBio8D9 reacts with an eptiope shared by mouse CD21 (CR2) and CD35 (CR1). CD21 and CD35 are alternatively spliced transcripts from the Cr2 gene, which produce cell-surface proteins of 145 and 190 kDa, respectively. CD21 and CD35 are expressed by mature B cells, but not on thymocytes, peripheral T cells, erythrocytes or platelets. Furthermore, there is some evidence which demonstrates their expression on macrophages. CD21 is a receptor for the complement component C3d and Epstein-Barr virus (EBV). In association with CD19 and CD81, CD21 also participates in B-cell activation through the B cell receptor. Cr2-deficient mice display impaired inflammatory and humoral immune responses in vivo.

The anti-mouse CD21/35 monoclonal antibody clones eBio4E3 and eBio8D9 do not cross-block each other, suggesting that they bind to different epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio8D9 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB). The eBio8D9 antibody can also be used in functional assays to crosslink CD21/CD35 both to itself and to sIgM. (Please use Functional Grade purified eBio8D9, cat. 16-0211, in functional assays.)

Applications Tested

This eBio8D9 (8D9) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kozono Y, Abe R, Kozono H, Kelly RG, Azuma T, and Holers VM. 1998. Cross-linking CD21/CD35 or CD19 increases both B7-1 and B7-2 expression on murine splenic B cells. Journal of Immunology. 160: 1565-1572. (8D9, FA, PubMed)

Martin, B.K., and J.H. Weis. 1993. Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products. Eur. J. Immunol. 23: 3037-3042.

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