human CD20 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-human CD20
Catalog Number: 14-0209
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 2H7
Isotype: Mouse IgG2b, κ
HLDA No.: IV B201

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14-0209-80

25 ug

14-0209-82

100 ug

Surface staining of normal human peripheral blood cells with anti-human CD20 (2H7) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0209	Affinity Purified anti-human CD20	N/A	N/A	FC IH/F
13-0209	Biotin anti-human CD20	N/A	N/A	FC IH/F
11-0209	FITC anti-human CD20	488	518	FC
12-0209	PE anti-human CD20	488	575	FC
15-0209	PE-Cy5 anti-human CD20	488	670	FC
17-0209	Allophycocyanin (APC) anti-human CD20	633	660	FC
25-0209	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD20	488	760	FC
56-0209	Alexa Fluor® 700 anti-human CD20 (APC-Cy5.5 Equivalent)	633	723	FC
45-0209	PerCP-Cy5.5 anti-human CD20 (PE-Cy5.5 replacement)	488	690	FC
94-0209	eFluor^TM 625^NC anti-human CD20	350-500	625	FC
95-0209	eFluor^TM 650^NC anti-human CD20	350-500	650	FC
47-0209	Allophycocyanin-eFluor^TM 780 anti-human CD20 (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0209	eFluor^TM 450 anti-human CD20	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 2H7 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The 2H7 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Reinherz, E.L., et al. eds. 1985. Leukocyte Typing II (Vol. I, II, and III). Human Leukocyte Differentiation Antigens detected by Monoclonal Antibodies.
McMichael, A.J., P.C.L. Beverly, et al. eds. 1987. Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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