Product Information

Contents: Affinity Purified anti-mouse CD20
Catalog Number: 14-0201
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: AISB12
Isotype: Rat IgG2a

(Left) Immunoblot of Balb/c thymus (lane 1) and A20 (lane 2) cell lysates with anti-mouse CD20 monoclonal antibody (clone: AISB12). (Right) Staining of BALB/c splenocytes with Alexa Fluor® 647 anti-mouse CD19 (1D3) (cat. 51-0193) and 1.0 μg Purified anti-mouse CD20 (AISB12) followed by PE Donkey anti-Rat IgG (cat. 12-4822) secondary antibody. Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0201	Phycoerythrin (PE) anti-mouse CD20	488	575	FC
14-0201	Affinity Purified anti-mouse CD20	N/A	N/A	FC WB

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Description

CD20 is a B cell-specific 32kDa membrane protein involved in intracellular calcium regulation. CD20 expression begins after the induction of CD19 during development, and is coexpressed with IgM during the pre-B to immature B cell transition in bone marrow. Expression then increases during maturation with almost all mature cells expressing some level of CD20. The human CD20 antigen has proven to be an effective immunotherapeutic target in B cell lymphoma and several autoimmune diseases including rheumatoid arthritis and type I diabetes. CD20 consists of 4 transmembrane domains and a 44 amino acid extracellular loop domain where AISB12 binds.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This AISB12 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB).

Applications Tested

This AISB12 antibody has been tested by immunoblot on A20 cell lysates and flow cytometry on mouse splenocytes.

References

Uchida J, Hamaguchi Y, Oliver JA, Ravetch JV, Poe JC, Haas KM, Tedder TF.The innate mononuclear phagocyte network depletes B lymphocytes through Fc receptor-dependent mechanisms during anti-CD20 antibody immunotherapy.J Exp Med. 2004 Jun 21;199(12):1659-69.

Uchida J, Lee Y, Hasegawa M, Liang Y, Bradney A, Oliver JA, Bowen K, Steeber DA, Haas KM, Poe JC, Tedder TF.Mouse CD20 expression and function.Int Immunol. 2004 Jan;16(1):119-29.

Tedder TF, Klejman G, Disteche CM, Adler DA, Schlossman SF, Saito H.Cloning of a complementary DNA encoding a new mouse B lymphocyte differentiation antigen, homologous to the human B1 (CD20) antigen, and localization of the gene to chromosome 19.J Immunol. 1988 Dec 15;141(12):4388-94.

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