14-0192

Contents: Affinity Purified anti-mouse CD19
Catalog Number: 14-0192
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 6D5
Isotype: Rat IgG2a, κ

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Staining of C57Bl/6 splenocytes with 0.06 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.06 μg of Purified anti-mouse CD19 (6D5) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications

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Description

The 6D5 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together, CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. 6D5 can be used as a phenotypic marker for mouse mature B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 6D5 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. It has also been reported in functional studies. (Please use Functional Grade purified 6D5 in functional assays).

Applications Tested

The 6D5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Krop, I., A. L. Shaffer, et al. (1996). "The signaling activity of murine CD19 is regulated during cell development." J Immunol 157(1): 48-56.
Krop, I., A. R. de Fougerolles, et al. (1996). "Self-renewal of B-1 lymphocytes is dependent on CD19." Eur J Immunol 26(1): 238-42.

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