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Mouse Cellular Antigens Chart

Contents: Affinity Purified anti-mouse CD19 Catalog Number: 14-0191 Formulation: Phosphate buffer pH 7.2, 500 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: MB19-1 Isotype: Mouse IgA, κ Prices for This Product* Cat. No. Size Price Qty Action 14-0191-81 50 ug $60 14-0191-82 100 ug $85 14-0191-85 500 ug $240 14-0191-86 1 mg $410 *International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 splenocytes with 1 μg of FG Purified Mouse IgA Iso Cntrl (cat. 16-4762) (open histogram) or 1 μg of FG Purified anti-mouse CD19 (MB19-1) (colored histogram) followed by FITC anti-mouse IgA (cat. 11-4204). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0191	Affinity Purified anti-mouse CD19	N/A	N/A	FA  FC  IP
16-0191	Functional Grade* Purified anti-mouse CD19	N/A	N/A	FA  FC  IP
13-0191	Biotin anti-mouse CD19	N/A	N/A	FC
11-0191	FITC anti-mouse CD19	488	518	FC
12-0191	PE anti-mouse CD19	488	575	FC
15-0191	PE-Cy5 anti-mouse CD19	488	670	FC
17-0191	APC anti-mouse CD19	633	660	FC
36-0191	Functional Grade* Biotin anti-mouse CD19	N/A	N/A	FA  FC  IP
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.   Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The MB19-1 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. Staining of B cells with MB19-1 and its conjugates is usually dimmer than the rat anti-mouse CD19 antibody, clone 6D5.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The MB19-1 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. MB19-1 has also been reported in in vitro functional studies. (Please use Functional Grade purified MB19-1 in functional assays.)

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Applications Tested

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The MB19-1 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Engel, P., L. J. Zhou, et al. (1995). "Abnormal B lymphocyte development, activation, and differentiation in mice that lack or overexpress the CD19 signal transduction molecule." Immunity 3(1): 39-50.
Sato, S., N. Ono, et al. (1996). "CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity." J Immunol 157(10): 4371-8.
Sato, S., D. A. Steeber, et al. (1997). "CD19 expression levels regulate B lymphocyte development: human CD19 restores normal function in mice lacking endogenous CD19." J Immunol 158(10): 4662-9.
Tedder, T. F., M. Inaoki, et al. (1997). "The CD19-CD21 complex regulates signal transduction thresholds governing humoral immunity and autoimmunity." Immunity 6(2): 107-18.

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Related Products

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Cat. 12-0192    PE anti-mouse CD19 (clone 6D5)
Cat. 14-0192    Affinity Purified anti-mouse CD19 (clone 6D5)
Cat. 15-0192    PE-Cy5 anti-mouse CD19 (clone 6D5)
Cat. 11-4204    Fluorescein isothiocyanate (FITC) anti-mouse IgA (clone mA-6E1)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4762    Affinity Purified Mouse IgA Isotype Control
Cat. 13-5994    Biotin anti-mouse IgA (clone 11-44-2)