Product Information

Contents: Affinity Purified anti-human CD18 (Integrin β₂)
Catalog Number: 14-0187
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: CLB-LFA-1/1
Isotype: Mouse IgG1
HLDA No.: II

Staining of normal human peripheral blood cells with 0.125 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.125 μg of Purified anti-human CD18 (CLB-LFA-1/1) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0187	Affinity Purified anti-human CD18 (Integrin beta 2)	N/A	N/A	FC
11-0187	Fluorescein isothiocyanate (FITC) anti-human CD18 (Integrin beta 2)	488	518	FC
12-0187	Phycoerythrin (PE) anti-human CD18 (Integrin beta 2)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The CLB-LFA-1/1 monoclonal antibody reacts with human CD18, also known as β₂ integrin. CD18 is a 90-95 kDa type I transmembrane protein and associates with the α chain of CD11a, CD11b and CD11c to make a heterodimer. The CD18/CD11a complex is also known as lymphocyte function-associated antigen-1 (LFA-1) and plays an important role in cellular adhesion and inflammatory reactions. CD18 is expressed broadly by human lymphocytes and monocytes. Peripheral blood granulocytes express CD18 at lower densities.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CLB-LFA-1/1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The CLB-LFA-1/1 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Reinherz, E. L., B. F. Haynes, L. M. Nadler, I. D. Bernstein eds. 1986. Leukocyte Typing II, Springer-Verlag Inc., New York.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control