Product Information

Contents: Affinity Purified anti-human CD14
Catalog Number: 14-0149
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 61D3
Isotype: Mouse IgG1, κ
HLDA No.: V MA085

Surface staining of normal human peripheral blood cells with anti-human CD14 (61D3) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0149	Affinity Purified anti-human CD14	N/A	N/A	FA FC IHC
16-0149	Functional Grade* Purified anti-human CD14	N/A	N/A	FA FC
13-0149	Biotin anti-human CD14	N/A	N/A	FC
11-0149	FITC anti-human CD14	488	518	FC
12-0149	PE anti-human CD14	488	575	FC
17-0149	APC anti-human CD14	633	660	FC
25-0149	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD14	488	760	FC
27-0149	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD14	633	779	FC
45-0149	PerCP-Cy5.5 anti-human CD14 (PE-Cy5.5 replacement)	488	690	FC
93-0149	eFluor^TM 605^NC anti-human CD14	350-500	605	FC
48-0149	eFluor^TM 450 anti-human CD14 (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 61D3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. 61D3 has also been reported in in vitro functional studies. (Please use Functional Grade purified 61D3, cat. 16-0149, in functional assays.)

Applications Tested

The 61D3 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.
Fadok VA, Warner ML, Bratton DL, Henson PM. CD36 is required for phagocytosis of apoptotic cells by human macrophages that use either a phosphatidylserine receptor or the vitronectin receptor (alpha v beta 3). J Immunol 1998 Dec 1;161(11):6250-7.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control