Product Information

Contents: Affinity Purified anti-mouse CD14
Catalog Number: 14-0141
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: Sa2-8
Isotype: Rat IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0141	Fluorescein (FITC) anti-mouse CD14	488	518	FC
12-0141	Phycoerythrin (PE) anti-mouse CD14	488	575	FC
13-0141	Biotin anti-mouse CD14	N/A	N/A	FC
14-0141	Affinity Purified anti-mouse CD14	N/A	N/A	FC IP WB
16-0141	Functional Grade* Purified anti-mouse CD14	N/A	N/A	FC
17-0141	Allophycocyanin (APC) anti-mouse CD14	633	660	FC
45-0141	PerCP-Cy5.5 anti-mouse CD14	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The Sa2-8 monoclonal antibody reacts with mouse CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is a receptor for the complexes of LPS and LBP (LPS-Binding Protein) and is shown to associate with Toll-Like Receptor 4 (TLR4) and participate in the signaling and cellular response to bacterial LPS. In mouse, CD14 is expressed on the surface of macrophages and under certain conditions is also found in the serum in a secreted form. Sa2-8 has weak antagonistic activity (in NF-κB activation or TNF-α production with LPS stimulation).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The Sa2-8 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).

Applications Tested

The Sa2-8 antibody has been tested by flow cytometric analysis of thioglycolate elicited PECs. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Akashi S, Saitoh S, Wakabayashi Y, Kikuchi T, Takamura N, Nagai Y, Kusumoto Y, Fukase K, Kusumoto S, Adachi Y, Kosugi A, Miyake K. 2003. Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14. J Exp Med. 198(7): 1035-42.

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