Product Information

Contents: Affinity Purified anti-human CD13
Catalog Number: 14-0138
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: WM-15 (WM15)
Isotype: Mouse IgG1, κ
HLDA No.: III M213

Staining of normal human peripheral blood cells with 0.5 µg of purified mouse IgG1 isotype control (cat.14-4714) (open histogram) or 0.5 µg of purified WM-15 (colored histogram) followed by FITC anti-mouse IgG (cat.11-4011). Cells in the monocyte (left) and granulocyte (right) gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0138	FITC anti-human CD13	488	518	FC
12-0138	PE anti-human CD13	488	575	FC MIC
17-0138	APC anti-human CD13	633	660	FC MIC
14-0138	Affinity Purified anti-human CD13	N/A	N/A	FC MIC
16-0138	Functional Grade* Purified anti-human CD13	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The WM-15 monoclonal antibody reacts with human CD13, also known as aminopeptidase N. CD13 is a transmembrane ectoenzyme occurring on monocytes and granulocytes but not lymphocytes in blood. It is also reportedly on a wide variety of cells and in a wide variety of tissues, including endothelial cells, epithelial cells, fibroblasts, kidney proximal tubules, intestine, and placenta. CD13 is reported to have many different functions including degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and adhesion and migration of cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The WM-15 (WM15) antibody) has been reported for use in flow cytometric analysis, and microscopy.

Applications Tested

The WM-15 (WM15) antibody has been tested by flow cytometric analysis of lysed whole blood. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Look AT, Ashmun RA, Shapiro LH, Peiper SC. 1989. Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N. J Clin Invest 83:1299-1307
Riemann D, Kehlen A, Thiele K, Lohn M, Langner J. 1997. Induction of aminopeptidase N/CD13 on human lymphocytes after adhesion to fibroblast-like synoviocytes, endothelial cells, epithelial cells, and monocytes/macrophages. J Immunol. 158(7):3425-32.

Related Products

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control