Affinity Purified anti-human CD11a (LFA-1α)

RUO: For Research Use Only

human CD11a antibody, Affinity Purified conjugate

Profile of purified mAb HI111 (CD11a) staining on human peripheral blood lymphocytes.

Contents: Affinity Purified anti-human CD11a (LFA-1α)
Catalog Number: 14-0119
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Clone: HI111
Host/Isotype: Mouse IgG1, κ
HLDA Workshop: IV N231
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
14-0119-80	25 ug	To view relevant pricing, please select your country and resume browsing.
14-0119-82	100 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-4011	FITC Anti-Mouse IgG
11-4317	Streptavidin-FITC (Fluorescein isothiocyanate)
12-4317	Streptavidin-PE (Phycoerythrin)
13-4013	Biotin Anti-Mouse IgG (Polyclonal)
14-4714	Affinity Purified Mouse IgG1, K Isotype Control
17-4317	Streptavidin Allophycocyanin (SA-APC)

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-0119	FITC anti-human CD11a	488	518	FC	RUO
12-0119	PE anti-human CD11a	488	575	FC	RUO
14-0119	Affinity Purified anti-human CD11a	N/A	N/A	FA FC IH/F	RUO
16-0119	Functional Grade* Purified anti-human CD11a	N/A	N/A	FA FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The HI111 monoclonal antibody reacts with human CD11a, the 180 kDa integrin α_L, also known as the lymphocyte function associated antigen-1 (LFA-1) α chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin β₂), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HI111 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. It has also been reported in blocking of CD11a function in mixed lymphocyte reactions. (Please use Functional Grade purified HI111, cat. 16-0119, in functional assays.)

Applications Tested

The HI111 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kapsogeorgou EK, Dimitriou ID, Abu-Helu RF, Moutsopoulos HM, Manoussakis MN. Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sjögren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells. Clin Exp Immunol. 2001 Apr;124(1):126-33. (HI111, IHC frozen)

Knapp, W., B. Dorken, et al. eds 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.