*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The HI111 monoclonal antibody reacts with human CD11a, the 180 kDa integrin αL, also known as the lymphocyte function associated antigen-1 (LFA-1) α chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin β2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This HI111 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. It has also been reported in blocking of CD11a function in mixed lymphocyte reactions. (Please use Functional Grade purified HI111, cat. 16-0119, in functional assays.)
Applications Tested
The HI111 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Kapsogeorgou EK, Dimitriou ID, Abu-Helu RF, Moutsopoulos HM, Manoussakis MN. Activation of epithelial and myoepithelial cells in the salivary glands of patients with Sjögren's syndrome: high expression of intercellular adhesion molecule-1 (ICAM.1) in biopsy specimens and cultured cells. Clin Exp Immunol. 2001 Apr;124(1):126-33. (HI111, IHC frozen)
Knapp, W., B. Dorken, et al. eds 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
