Product Information

Contents: Affinity Purified anti-human CD11b (Integrin α_M, Mac-1α)
Catalog Number: 14-0118
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: ICRF44
Isotype: Mouse IgG1, κ
HLDA No.: IV M047

Surface staining of normal human peripheral blood cells with anti-human CD11b (ICRF44) PE. Autofluorescence is indicated by open histogram. Cells in the monocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0118	Affinity Purified anti-human CD11b (Integrin alpha M, Mac-1 alpha)	N/A	N/A	FA FC IHC
13-0118	Biotin anti-human CD11b (Integrin alpha M, Mac-1 alpha)	N/A	N/A	FC
11-0118	Fluorescein isothiocyanate (FITC) anti-human CD11b (Integrin alpha M, Mac-1 alpha)	488	518	FC
12-0118	PE anti-human CD11b (Integrin alpha M, Mac-1 alpha)	488	575	FC
17-0118	Allophycocyanin (APC) anti-human CD11b (Integrin alpha M, Mac-1 alpha)	633	660	FC
25-0118	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD11b (Integrin alpha M, Mac-1 alpha)	488	760	FC
93-0118	eFluor^TM 605^NC anti-human CD11b(Integrin alpha M, Mac-1 alpha)	350-500	605	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin β₂ (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The ICRF44 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. ICRF44 has also been reported in inhibition of aggregation of PMNs. (Please use Functional Grade purified MM2/57 in functional assays.)

Applications Tested

The ICRF44 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-0112    FITC anti-mouse CD11b (Integrin alpha M, Mac-1 alpha) (clone M1/70)
Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control